Scientific Papers

  • 2017

  • Huter P, Müller C, Beckert B, Arenz S, Berninghausen O, Beckmann R, Wilson DN.

    Structural basis for ArfA–RF2-mediated translation termination on mRNAs lacking stop codons

    Nature (2017) Jan 26;541(7638):546-549

  • 2016

  • Graf M, Arenz S, Huter P, Dönhöfer A, Nováček J, Wilson DN.

    Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions.

    Nucleic Acids Res. 2016 Dec 15. pii: gkw1272. [Epub ahead of print]

  • Prunetti L, Graf M, Blaby IK, Peil L, Makkay AM, Starosta AL, Papke RT, Oshima T, Wilson DN and de Crécy-

    Lagard V.

    Deciphering the Translation Initiation Factor 5A Modification Pathway in Halophilic Archaea

    Archaea, (2016) Dec 8;2016:7316725. doi: 10.1155/2016/7316725..

  • Wilson DN and Clark PL.

    Climbing to the peak of nascent-chain knowledge.

    Nat Struct Mol Biol. 2016 Nov 4;23(11):949-951.

  • Arenz S and Wilson DN

    Bacterial Protein Synthesis as a Target for Antibiotic Inhibition

    Cold Spring Harb Perspect Med, 2016 Sep 1;6(9).

  • Arenz S, Bock LV, Graf M, Innis CA, Beckmann R, Grubmüller H, Vaiana AC, Wilson DN.

    A combined cryo-EM and molecular dynamics approach reveals the mechanism of ErmBL-mediated translation arrest.

    Nat Commun. 2016 Jul 6;7:12026. doi: 10.1038/ncomms12026.

  • Arenz S, Juette MF, Graf M, Nguyen F, Huter P, Polikanov YS, Blanchard SC, Wilson DN.

    Structures of the orthosomycin antibiotics avilamycin and evernimicin in complex with the bacterial 70S ribosome.

    Proc Natl Acad Sci USA. 2016, Jul 5;113(27):7527-32.


  • Wilson DN, Spahn CM.

    Knud Hermann Nierhaus 1941-2016.

    Nat Struct Mol Biol. 2016 Jun 7;23(6):503-4. doi: 10.1038/nsmb.3239.


  • Wilson DN, Spahn C, Yusupov M.

    Ribosomes Structure and Mechanisms in Regulation of Protein Synthesis Part I.

    J Mol Biol. 2016 May 22;428(10 Pt B):2133.


  • Arenz S, Abdelshahid M, Sohmen D, Payoe R, Starosta AL, Berninghausen O, Hauryliuk V, Beckmann R, Wilson DN.

    The stringent factor RelA adopts an open conformation on the ribosome to stimulate ppGpp synthesis.

    Nucleic Acids Res. 2016, Jul 27;44(13):6471-81


  • Daniel N. Wilson

    The ABC of Ribosome-Related Antibiotic Resistance

    mBio 7(3): e00598-16.

  • Wilson DN, Arenz S, Beckmann R

    Translation regulation via nascent polypeptide-mediated ribosome stalling

    Current Opinion in Structural Biology 2016, 37:123–133.

  • Seefeldt CA, Graf M, Pérébaskine N, Nguyen F, Arenz S, Mardirossian M, Scocchi M, Wilson DN* and Innis CA*

    Structure of the mammalian antimicrobial peptide Bac7(1- 16) bound within the exit tunnel of a bacterial ribosome

    Nucleic Acids Res., 2016, Mar 18;44(5):2429-38.


  • Schmidt C, Becker T, Heuer A, Braunger K, Shanmuganathan V, Pech M, Berninghausen O, Wilson DN* and Beckmann R*

    Structure of the hypusinylated eukaryotic translation factor eIF-5A bound to the ribosome

    Nucleic Acids Res., 2016, Feb 29;44(4):1944-51.


  • Lassak J, Wilson DN, Jung K.

    Stall no more at polyproline stretches with the translation factors EF-P and IF-5A.

    Mol Microbiol. 2016 Jan; 99(2), 219-235.

  • Arenz S, Wilson DN.

    Blast from the Past: Reassessing Forgotten Translation Inhibitors, Antibiotic Selectivity, and Resistance Mechanisms to Aid Drug Development.

    Mol Cell. 2016 Jan 7;61(1):3-14.

  • 2015

  • Doerfel LK, Wohlgemuth I, Kubyshkin V, Starosta AL, Wilson DN, Budisa N, Rodnina MV.

    Entropic contribution of elongation factor P to proline positioning at the catalytic center of the ribosome.

    J Am Chem Soc. 2015 Oct 14;137(40):12997-3006.

  • Beckert B, Kedrov A, Sohmen D, Kempf G, Wild K, Sinning I, Stahlberg H, Wilson DN, Beckmann R.

    Translational arrest by a prokaryotic signal recognition particle is mediated by RNA interactions.

    Nat Struct Mol Biol. 2015 Oct;22(10):767-73.

  • Wilson DN, Guichard G, Innis CA.

    Antimicrobial peptides target ribosomes.

    Oncotarget. 2015 Jul 10;6(19):16826-7.

  • Polikanov YS, Starosta AL, Juette MF, Altman RB, Terry DS, Lu W, Burnett BJ, Dinos G, Reynolds K, Blanchard SC, Steitz TA, Wilson DN

    Distinct tRNA accommodation intermediates observed on the ribosome with the antibiotics hygromycin A and A201A.

    Molecular Cell, 2015, Jun 4;58(5):832-44.

  • Seefeldt AC, Nguyen F, Antunes S, Pérébaskine N, Graf M, Arenz S, Inampudi KK, Douat C, Guichard G, Wilson DN, Innis CA

    The proline-rich antimicrobial peptide Onc112 inhibits translation by blocking and destabilizing the initiation complex.

    Nat. Struct. Mol Biol. 2015 Jun;22(6):470-5.

  • Sohmen D, Chiba S, Shimokawa-Chiba N, Innis CA, Berninghausen O, Beckmann R, Ito K and Wilson DN

    Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling.

    Nature communications, 2015 Apr 23;6:6941. doi: 10.1038/ncomms7941.

  • Arenz S, Nguyen F, Beckmann R and Wilson DN

    Cryo-EM structure of the tetracycline resistance protein TetM in complex with a translating ribosome at 3.9 Å resolution.

    Proc. Natl Acad. Sci. USA, 2015 Apr 28;112(17):5401-6.

  • Lassak J, Keilhauer E, Fürst M, Wuichet K, Gödeke J, Starosta AL, Chen JM, Søgaard-Andersen L, Rohr J, Wilson DN, Häussler S, Mann M, Jung K.

    Arginine-rhamnosylation as new strategy to activate translation elongation factor P.

    Nat Chem Biol. 2015 Apr;11(4):266-70.

  • Byrgazov K, Grishkovskaya I, Arenz S, Coudevylle N, Temmel H, Wilson DN, Djinovic-Carugo K and Moll I

    Structural basis for the interaction of protein S1 with the Escherichia coli ribosome

    Nucleic acids Res., Jan 9;43(1):661-73.

  • 2014

  • Arenz S, Meydan S, Starosta AL, Berninghausen O, Beckmann R, Vázquez-Laslop N, Wilson DN

    Drug-Sensing by the Ribosome Induces Translational Arrest via Active Site Perturbation

    Molecular Cell, 2014, Nov 6, 56(3), 446–452.


  • Starosta AL, Lassak J, Peil L, Atkinson GC, Woolstenhulme CJ, Virumäe K, Buskirk A, Tenson T, Remme J, Jung K, Wilson DN.

    A conserved proline triplet in Val-tRNA synthetase and the origin of elongation factor P

    Cell reports, 2014 Oct 23;9(2):476-83.


  • Starosta AL, Lassak J, Peil L, Atkinson G, Virumäe K, Tenson T, Remme J, Jung K, Wilson DN

    Translational stalling at polyproline-stretches is modulated by the sequence context upstream of the stall site

    Nucleic Acids Res., Dec 1;42(16):10711-9.


  • Starosta AL, Lassak J, Jung K and Wilson DN

    The bacterial translation stress response

    FEMS Microbiology Rev., 2014 Nov;38(6):1172-201


  • Arenz S, Ramu H, Gupta P, Berninghausen O, Beckmann R, Vázquez-Laslop N, Mankin AS & Wilson DN

    Molecular basis for erythromycin-dependent ribosome stalling during translation of the ErmBL leader peptide

    Nature Communications, 2014, Mar 24;5:3501.


  • Ghalei H, von Moeller H, Eppers D, Sohmen S, Wilson DN, Loll B and Wahl MC.

    Oligomerization and DNA binding in non-canonical single-stranded DNA binding proteins from archaea

    Nucleic Acids Res. 2014, 42(10):6698-708.


  • Starosta AL and Wilson DN

    Ribosome Rescue, Nearing the End

    Cell 2014, 156(5):866-867.


  • Ban N, Beckmann R, Cate JH, Dinman JD, Dragon F, Ellis SR, Lafontaine DL, Lindahl L, Liljas A, Lipton JM, McAlear MA, Moore PB, Noller HF,Ortega J, Panse VG, Ramakrishnan V, Spahn CM, Steitz TA, Tchorzewski M, Tollervey D, Warren AJ, Williamson JR, Wilson D, Yonath A,Yusupov M.

    A new system for naming ribosomal proteins.

    Curr Opin Struct Biol. 2014 Feb;24C:165-169.


  • Wilson DN.

    Ribosome-targeting antibiotics and mechanisms of bacterial resistance.

    Nat Rev Microbiol. 2014, 12(1):35-48.


  • Nguyen F, Starosta AL, Arenz S, Sohmen D, Dönhöfer A, Wilson DN.

    Tetracycline antibiotics and resistance mechanisms.

    Biol. Chem. 2014 May 1;395(5):559-75


  • Krokidis MG, Márquez V, Wilson DN, Kalpaxis DL, Dinos GP.

    Insights into the mode of action of novel fluoroketolides, potent inhibitors of bacterial protein synthesis.

    Antimicrob Agents Chemother. 2014, 58(1):472-80


  • 2013

  • Peil L, Starosta AL, Lassak J, Atkinson GC, Virumäe K, Spitzer M, Tenson T, Jung K, Remme J, Wilson, DN

    Distinct XPPX sequence motifs induce ribosome stalling, which is rescued by the translation elongation factor EF-P

    Proc. Natl. Acad. Sci. USA, 2013, 110(38):15265-70


  • Anger AM, Armache JP, Berninghausen O, Habeck M, Subklewe M, Wilson DN and Beckmann R

    Structures of the human and Drosophila 80S ribosome

    Nature, 2013 May 2, 497(7447): 80-85.


  • Gross S, Nguyen F, Bierschenk M, Sohmen D, Menzel T, Antes I, Wilson DN, and Bach T

    Amythiamicin D and related thiopeptides as inhibitors of the bacterial elongation factor EF-Tu: Modification of the amino acid at carbon atom C2 of ring C dramatically influences activity

    ChemMedChem, 2013 May 17, 8(12):1954-62.


  • Jenner L, Starosta AL, Terry DS, Mikolajka A, Filonava L, Yusupov M, Blanchard SC, Wilson DN and Yusupova G

    Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis

    Proc. Natl. Acad. Sci. USA, 2013 Mar 5;110(10):3812-6.


  • Mamos P, Krokidis MG, Papadas A, Karahalios P, Starosta AL, Wilson DN, Kalpaxis DL, Dinos GP.

    On the use of the antibiotic chloramphenicol to target polypeptide chain mimics to the ribosomal exit tunnel.

    Biochimie, 2013 Sep;95(9):1765-72.


  • Woolstenhulme CJ, Parajuli S, Healey DW, Valverde DP, Petersen EN, Starosta AL, Guydosh NR, Johnson WE, Wilson DN and Buskirk AR

    Nascent peptides that block protein synthesis in bacteria

    Proc. Natl. Acad. Sci. USA, 2013 Mar 5;110(10):E878-87.


  • Ude S, Lassak J, Starosta AL, Kraxenberger T, Wilson DN, Jung K.

    Translation elongation factor EF-P alleviates ribosome stalling at polyproline stretches.

    Science, 339(6115):82-85.


  • Armache JP, Anger AM, Marquez V, Franckenberg S, Fröhlich T, Villa E, Berninghausen O, Thomm M, Arnold GJ, Beckmann R, Wilson DN

    Promiscuous behaviour of archaeal ribosomal proteins: Implications for eukaryotic ribosome evolution

    Nucleic Acids Res. 41(2):1284-1293.


  • 2012

  • Dönhöfer A, Franckenberg S, Wickles S, Berninghausen O, Beckmann R and Wilson DN

    Structural basis for TetM-mediated tetracycline resistance

    Proc. Natl. Acad. Sci. USA, 109(42):16900-5


  • Gumbart J, Schreiner E, Wilson DN, Beckmann R and Schulten K

    Mechanisms of SecM-Mediated Stalling in the Ribosome

    Biophysical Journal, 2012, 103:331–341


  • Peil L, Starosta AL, Virumäe K, Atkinson GC, Tenson T, Remme J, Wilson DN

    Lysine 34 of translation elongation factor EF-P is hydroxylated by YfcM

    Nature Chem Biol, 8(8): 695-697.


  • Wilson DN and Cate JHD

    The structure and function of the eukaryotic ribosome

    Cold Spring Harb Perspect Biol, 2012; May 1;4(5): 1-17.


  • Grossman TH, Starosta AL, Fyfe C, O'Brien W, Rothstein DM, Mikolajka A, Wilson DN, Sutcliffe JA.

    Target- and resistance-based mechanistic studies with TP-434, a novel fluorocycline antibiotic.

    Antimicrob Agents Chemother. 2012 May;56(5):2559-64.


  • Jarasch A, Dziuk P, Becker T, Armache JP, Hauser A, Wilson DN, Beckmann R.

    The DARC site: a database of aligned ribosomal complexes.

    Nucleic Acids Res. 2012 Jan, 40(Database issue):D495-500.


  • 2011

  • Wilson DN

    On the specificity of antibiotics targeting the large ribosomal subunit

    Ann N Y Acad Sci. 2011 Dec;1241(1):1-16.


  • Akbergenov R, Shcherbakov D, Matt T, Duscha S, Meyer M, Wilson DN, Böttger EC

    Molecular basis for the selectivity of antituberculosis compounds capreomycin and viomycin

    Antimicrob Agents Chemother, 55:4712-7


  • Mikolajka A, Liu H, Chen Y, Starosta AL, Márquez V, Ivanova M, Cooperman BS, Wilson DN

    Differential effects of thiopeptide and orthosomycin antibiotics on translational GTPases.

    Chem Biol. 18: 589-600.


  • Siibak T, Peil L, Dönhöfer A, Tats A, Remm M, Wilson DN, Tenson T, Remme J

    Antibiotic-induced ribosomal assembly defects result from changes in the synthesis of ribosomal proteins.

    Mol Microbiol. 80: 54-67


  • Wilson DN and Beckmann R

    The ribosomal tunnel as a functional environment for nascent polypeptide folding and translational stalling

    Curr. Opin. Struct. Biol. 21: 274-282.


  • Wilson DN

    Peptides in the ribosomal tunnel talk back.

    Mol. Cell, 41(3):247-248. Preview.


  • Bhushan S, Hoffmann T, Seidelt B, Frauenfeld J, Mielke T, Berninghausen O, Wilson DN and Beckmann R

    SecM-Stalled ribosomes adopt an altered geometry at the peptidyltransferase center.

    PLoS Biol. 9: e1000581.


  • Márquez V, Fröhlich T, Armache JP, Sohmen D, Dönhöfer A, Mikolajka A, Berninghausen O, Thomm M, Beckmann R, Arnold GJ, Wilson DN

    Proteomic characterization of archaeal ribosomes reveals the presence of novel archaeal-specific ribosomal proteins

    J Mol Biol. 405: 1215-1232


  • 2010

  • Ratje AH, Loerke J, Mikolajka A, Brünner M, Hildebrand PW, Starosta AL, Dönhöfer A, Connell SR, Fucini P, Mielke T, Whitford PC, Onuchic JN, Yu Y, Sanbonmatsu KY, Hartmann RK, Penczek PA, Wilson DN, and Spahn CMT

    Head swivel on the ribosome facilitates translocation via intra-subunit tRNA hybrid sites.

    Nature, 468:713-6.


  • Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Soeding J, Westhof E, Wilson DN and Beckmann R

    Localization of eukaryote-specific ribosomal proteins in a 5.5 Å cryo-EM map of the 80S eukaryotic ribosome.

    Proc. Natl. Acad. Sci. USA, 107:19754-9.


  • Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Soeding J, Westhof E, Wilson DN and Beckmann R

    Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5 Å resolution

    Proc. Natl. Acad. Sci. USA, 107:19748-53


  • Bhushan S, Meyer H, Starosta AL, Becker T, Mielke T, Berninghausen O, Sattler M, Wilson DN, Beckmann R

    Structural basis for translational stalling by human cytomegalovirus and fungal arginine attenuator peptide.

    Molecular Cell, 40:138-46.


  • Blanchard SC, Cooperman BS, Wilson DN

    Probing translation with small molecule inhibitors.

    Chem. Biol. 17:633-45.


  • Starosta AL, Karpenko V, Shishkina A, Mikolajka A, Sumbatyan N, Schluenzen F, Korshunova G, Bogdanov A, Wilson DN

    Interplay between the ribosomal tunnel, nascent chain, and macrolides influences drug inhibition

    Chem Biol 17: 504-14.


  • Bhushan S, Gartmann M, Halic M, Armache JP, Jarasch A, Mielke T, Berninghausen O, Wilson DN, Beckmann R

    alpha-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel.

    Nat Struct Mol Biol 17: 313-317.


  • Sharma MR, Donhofer A, Barat C, Marquez V, Datta PP, Fucini P, Wilson DN, Agrawal RK

    PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the ribosome-recycling factor (RRF) and elongation factor G (EF-G).

    J Biol Chem 285: 4006-4014.


  • 2009

  • Palaniappan N, Dhote V, Ayers S, Starosta AL, Wilson DN, Reynolds KA

    Biosynthesis of the aminocyclitol subunit of hygromycin A in Streptomyces hygroscopicus NRRL 2388.

    Chem Biol 16: 1180-1189.


  • Seidelt B, Innis CA, Wilson DN, Gartmann M, Armache JP, Villa E, Trabuco LG, Becker T, Mielke T, Schulten K, Steitz TA, Beckmann R

    Structural insight into nascent polypeptide chain-mediated translational stalling.

    Science 326: 1412-1415.


  • Wilson DN

    The A-Z of bacterial translation inhibitors.

    Crit Rev Biochem Mol Biol 44: 393-433.


  • Starosta AL, Qin H, Mikolajka A, Leung GY, Schwinghammer K, Nicolaou KC, Chen DY, Cooperman BS, Wilson DN

    Identification of distinct thiopeptide-antibiotic precursor lead compounds using translation machinery assays.

    Chem Biol 16: 1087-1096.


  • Sohmen D, Harms JM, Schlunzen F, Wilson DN

    Enhanced SnapShot: Antibiotic inhibition of protein synthesis II.

    Cell 139: 212-212 e211.


  • Dhote V, Starosta AL, Wilson DN, Reynolds KA

    The final step of hygromycin A biosynthesis, oxidation of C-5''-dihydrohygromycin A, is linked to a putative proton gradient-dependent efflux.

    Antimicrob Agents Chemother 53: 5163-5172.


  • Sohmen D, Harms JM, Schlunzen F, Wilson DN

    SnapShot: Antibiotic inhibition of protein synthesis I.

    Cell 138: 1248 e1241.


  • Moroder H, Steger J, Graber D, Fauster K, Trappl K, Marquez V, Polacek N, Wilson DN, Micura R

    Non-hydrolyzable RNA-peptide conjugates: a powerful advance in the synthesis of mimics for 3'-peptidyl tRNA termini.

    Angew Chem Int Ed Engl 48: 4056-4060.


  • Wilson DN

    Less is more for leaderless mRNA translation.

    Mol Cell 33: 141-142.


  • Kouvela EC, Kalpaxis DL, Wilson DN, Dinos GP

    Distinct mode of interaction of a novel ketolide antibiotic that displays enhanced antimicrobial activity.

    Antimicrob Agents Chemother 53: 1411-1419.


  • Petropoulos AD, Kouvela EC, Starosta AL, Wilson DN, Dinos GP, Kalpaxis DL

    Time-resolved binding of azithromycin to Escherichia coli ribosomes.

    J Mol Biol 385: 1179-1192.


  • 2008

  • Connell SR, Topf M, Qin Y, Wilson DN, Mielke T, Fucini P, Nierhaus KH, Spahn CM

    A new tRNA intermediate revealed on the ribosome during EF4-mediated back-translocation.

    Nat Struct Mol Biol 15: 910-915.


  • Wilson DN, Schluenzen F, Harms JM, Starosta AL, Connell SR, Fucini P

    The oxazolidinone antibiotics perturb the ribosomal peptidyl-transferase center and effect tRNA positioning.

    Proc Natl Acad Sci U S A 105: 13339-13344.


  • Di Giacco V, Marquez V, Qin Y, Pech M, Triana-Alonso FJ, Wilson DN, Nierhaus KH

    Shine-Dalgarno interaction prevents incorporation of noncognate amino acids at the codon following the AUG.

    Proc Natl Acad Sci U S A 105: 10715-10720.


  • Szaflarski W, Vesper O, Teraoka Y, Plitta B, Wilson DN, Nierhaus KH

    New features of the ribosome and ribosomal inhibitors: non-enzymatic recycling, misreading and back-translocation.

    J Mol Biol 380: 193-205.


  • Lang K, Erlacher M, Wilson DN, Micura R, Polacek N

    The role of 23S ribosomal RNA residue A2451 in peptide bond synthesis revealed by atomic mutagenesis.

    Chem Biol 15: 485-492.


  • Harms JM, Wilson DN, Schluenzen F, Connell SR, Stachelhaus T, Zaborowska Z, Spahn CM, Fucini P

    Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin.

    Mol Cell 30: 26-38.


  • 2007

  • Sharma MR, Wilson DN, Datta PP, Barat C, Schluenzen F, Fucini P, Agrawal RK

    Cryo-EM study of the spinach chloroplast ribosome reveals the structural and functional roles of plastid-specific ribosomal proteins.

    Proc Natl Acad Sci U S A 104: 19315-19320.


  • Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK

    Structural aspects of RbfA action during small ribosomal subunit assembly.

    Mol Cell 28: 434-445.


  • Wilson DN, Nierhaus KH

    The weird and wonderful world of bacterial ribosome regulation.

    Crit Rev Biochem Mol Biol 42: 187-219.


  • Wilson DN, Nierhaus KH

    The oxazolidinone class of drugs find their orientation on the ribosome.

    Mol Cell 26: 460-462.


  • Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S

    A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction.

    Structure 15: 289-297


  • Connell SR, Takemoto C, Wilson DN, Wang H, Murayama K, Terada T, Shirouzu M, Rost M, Schuler M, Giesebrecht J, Dabrowski M, Mielke T, Fucini P, Yokoyama S, Spahn CM

    Structural basis for interaction of the ribosome with the switch regions of GTP-bound elongation factors.

    Mol Cell 25: 751-764.


  • 2006

  • Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN, Nierhaus KH

    The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome.

    Cell 127: 721-733.


  • Wilson DN, Nierhaus KH

    The E-site Story: The importance of maintaining two tRNAs on the ribosome during protein synthesis.

    Cell Mol Life Sci 63: 2725-2737.

  • Schluenzen F, Takemoto C, Wilson DN, Kaminishi T, Harms JM, Hanawa-Suetsugu K, Szaflarski W, Kawazoe M, Shirouzu M, Nierhaus KH, Yokoyama S, Fucini P

    The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation.

    Nat Struct Mol Biol 13: 871-878.


  • Vesper O, Wilson DN

    Ribosome recycling revisited.

    Mol Biol (Moscow) 40: 742-750.

  • Karahalios P, Kalpaxis DL, Fu H, Katz L, Wilson DN, Dinos GP

    On the mechanism of action of 9-O-arylalkyloxime derivatives of 6-O-mycaminosyltylonolide, a new class of 16-membered macrolide antibiotics.

    Mol Pharmacol 70: 1271-1280.


  • Seo H, Abedin S, Kamp D, Wilson DN, Nierhaus KH, Cooperman BS

    EF-G-dependent GTPase on the ribosome. Conformational change and fusidic acid inhibition.

    Biochemistry 45: 2504-2514.


  • 2005

  • Wilson DN, Harms JM, Nierhaus KH, Schlünzen F, Fucini P

    Species-specific antibiotic-ribosome interactions: Implications for drug development.

    Biol Chem 386: 1239-1252.


  • Schlünzen F, Wilson DN, Tian P, Harms JM, McInnes SJ, Hansen HA, Albrecht R, Buerger J, Wilbanks SM, Fucini P

    The binding mode of the trigger factor on the ribosome: implications for protein folding and SRP interaction.

    Structure (Camb) 13: 1685-1694.


  • Wilson DN, Nierhaus KH

    Ribosomal Proteins in the Spotlight.

    Crit Rev Biochem Mol Biol 40: 243-267.


  • Dinos G, Kalpaxis DL, Wilson DN, Nierhaus KH

    Deacylated tRNA is released from the E site upon A site occupation but before GTP is hydrolyzed by EF-Tu.

    Nucleic Acids Res 33: 5291-5296.


  • Sharma MR, Barat C, Wilson DN, Booth TM, Kawazoe M, Hori-Takemoto C, Shirouzu M, Yokoyama S, P. F, Agrawal RK

    Interaction of Era with the 30S Ribosomal Subunit: Implications for 30S Subunit Assembly.

    Mol Cell 18: 319-329.


  • Wilson DN and Nierhaus KH

    RelBE or not to be.

    Nat Struct Mol Biol 12: 282-284.


  • Giavalisco P, Wilson DN, Kreitler T, Lehrach H, Klose J, Gobom J, Fucini P

    High heterogeneity within the ribosomal proteins of the Arabidopsis thaliana 80S ribosome.

    Plant Mol Biol 57: 577-591.


  • Wilson DN, Schluenzen F, Harms JM, Yoshida T, Ohkubo T, Albrecht R, Buerger J, Kobayashi Y, Fucini P

    X-ray crystallography study on ribosome recycling: the mechanism of binding and action of RRF on the 50S ribosomal subunit.

    EMBO J 24: 251-260.


  • 2004

  • Wilson DN, Nierhaus KH

    The how and Y of cold shock.

    Nat Struct Mol Biol 11: 1026-1028.

  • Marquez V, Wilson DN, Tate WP, Triana-Alonso F, Nierhaus KH

    Maintaining the ribosomal reading frame: The influence of the E site during translational regulation of release factor 2.

    Cell 118: 45-55.


  • Petropoulos AD, Xaplanteri MA, Dinos GP, Wilson DN, Kalpaxis DL

    Polyamines affect diversely the antibiotic potency: insight gained from kinetic studies of the blasticidin S and spiramycin interactions with functional ribosomes.

    J Biol Chem 279: 26518-26525.

  • Dinos G, Wilson DN, Teraoka Y, Szaflarski W, Fucini P, Kalpaxis D, Nierhaus KH

    Dissecting the ribosomal inhibition mechanisms of edeine and pactamycin: the universally conserved residues G693 and C795 regulate P-site tRNA binding.

    Mol Cell 13: 113-124.


  • Sharma MR, Barat C, Wilson DN, Booth TM, Hori-Takemoto C, Yokoyama S, Fucini P, and Agrawal, RK

    ERA protein binds in a functionally important region of the 30S ribosomal subunit

    Biophys. J.  86: 316A-317A (Part 2 Suppl. S).

  • 2003

  • Poole ES, Askarian-Amiri ME, Major LL, McCaughan KK, Scarlett DJ, Wilson DN, Tate WP

    Molecular mimicry in the decoding of translational stop signals.

    Prog Nucleic Acid Res Mol Biol 74: 83-121.

  • Bashan A, Zarivach R, Schluenzen F, Agmon I, Harms J, Auerbach T, Baram D, Berisio R, Bartels H, Hansen H, Fucini P, Wilson DN, Peretz M, Kessler M, Yonath A

    Ribosomal Crystallography: peptide bond formation and its inhibition.

    Biopolymers 79: 19-41.

  • Wilson DN, Nierhaus KH

    The ribosome through the looking glass.

    Angew Chem Int Ed Engl 42: 3464-3486.


  • Blaha G, Wilson DN, Stoller G, Fischer G, Willumeit R, Nierhaus KH

    Localization of the trigger factor binding site on the ribosomal 50S subunit.

    J Mol Biol 326: 887-897.


  • Scarlett DJ, McCaughan KK, Wilson DN, Tate WP

    Mapping functionally important motifs SPF and GGQ of the decoding release factor RF2 to the Escherichia coli ribosome by hydroxyl radical footprinting. Implications for macromolecular mimicry and structural changes in RF2.

    J Biol Chem 278: 15095-15104.

  • up to 2002

  • Wendrich TM, Blaha G, Wilson DN, Marahiel MA, Nierhaus KH

    Dissection of the mechanism for the stringent factor RelA.

    Mol Cell 10: 779-788.


  • Wilson DN, Blaha G, Connell SR, Ivanov PV, Jenke H, Stelzl U, Teraoka Y, Nierhaus KH

    Protein synthesis at atomic resolution: mechanistics of translation in the light of highly resolved structures for the ribosome.

    Curr Protein Pept Sci 3: 1-53.

  • Márquez V, Wilson DN, Nierhaus KN

    Functions and interplay of the tRNA-binding sites of the ribosome.

    Biochemical Society Transactions 30: 133-140.

  • Schäfer MA, Tastan AO, Patzke S, Blaha G, Spahn CM, Wilson DN, Nierhaus KH

    Codon-anticodon interaction at the P Site is a prerequisite for tRNA interaction with the small ribosomal subunit.

    J Biol Chem 277: 19095-19105.

  • Wilson DN, Guevremont D, Tate WP

    The ribosomal binding and peptidyl-tRNA hydrolysis functions of Escherichia coli release factor 2 are linked through residue 246.

    RNA 6: 1704-1713.

  • Tate WP, Mansell JB, Mannering SA, Irvine JH, Major LL, Wilson DN

    UGA: a dual signal for 'stop' and for recoding in protein synthesis.

    Biochemistry (Mosc) 64: 1342-1353.

  • Horsfield JA, Wilson DN, Mannering SA, Adamski FM, Tate WP

    Prokaryotic ribosomes recode the HIV-1 gag-pol-1 frameshift sequence by an E/P site post-translocation simultaneous slippage mechanism.

    Nucleic Acids Res 23: 1487-1494.

  • Tate WP, Poole ES, Horsfield JA, Mannering  SA, Brown CM, Moffat JG, Dalphin ME, McCaughan KK, Major LL, Wilson DN

    Translational termination efficiency in both bacteria and mammals is regulated by the base following the stop codon.

    Biochemistry & Cell Biology 73: 1095-1103.

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