2. Translational response to environmental stress

Additional factors and regulation systems appear to be particularly important for efficient translation in response to changes in environmental conditions. Many have been well-characterized systems include the RelA-mediated stringent response and tmRNA-mediated rescue systems that are activated in response to nutrient deprivation. In addition numerous factors bind and protect the ribosome upon entering stationary phase, such as RMF, SRA, YfiA and YhbH, or under conditions of cold shock and shifts in pH and/or ionic strength, such as protein Y and LepA, respectively. Indeed, elongation factor P (EFP) has been shown to be important for virulence in Salmonella enterica, suggesting that insight into how such factors interact with the ribosome to modulate translation could have important implications for development of novel antimicrobial agents. The Wilson lab is currently investigating the mechanistic link by which binding of factors to the ribosome can influence translation in response to a variety of different stress conditions, ranging from antibiotic sensitivity to nutrient deprivation.